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| 論文分類 | 碩士論文 |
|---|---|
| 學號 | 9025614 |
| 姓名 | 吳彩楣 |
| 標題(中) | 實驗計畫法應用於污泥DNA萃取與PCR之研究 |
| 標題(英) | A Design of Experiment to DNA Extraction and PCR on Sludge |
| 指導教授 | 莊順興 |
| 畢業日期 | 2003-07 |
| 附件檔案 | |
| 參考連結 | http://ir.lib.cyut.edu.tw:8080/handle/310901800/1684 |
| 摘要 | 應用分子生物技術，可不需經由分離培養而直接針對微生物核酸進行分子序列之分析，解決傳統鑑別微生物之缺點。而聚合酵素連鎖反應 ( Polymerase Chain Reaction PCR )，是種迅速、無須培養，直接利用引子 ( primer ) 複製目標微生物特定基因序列之體外複製技術，藉由複製其特殊之DNA片段，進而鑑定微生物的屬、種及基因型。 過去許多研究針對不同的環境樣品，以不同的方法溶解微生物細胞，欲萃取其核酸物質以進行PCR，進而分析微生物之族群。污泥之DNA萃取為分子生物分析最基本、最重要之環 |
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